Raman spectroscopic probing of plant material using SERS. (24th August 2015)

• Record Type: Journal Article
• Authors: Espina Palanco, Marta; Mogensen, Klaus Bo; Kneipp, Katrin
• Is Part Of: Journal of Raman spectroscopy. Volume 47:Number 2(2016)
• Page Start: 156
• Page End: 161
• Publication Date: 2015-08-24
• Subjects: surface‐enhanced Raman spectroscopy -- silver nanoparticles -- silver island films -- plant material -- onion -- pH sensor; Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.4768
• Languages: English
• ISSNs: 0377-0486

Abstract

Abstract : We report surface‐enhanced Raman studies on intact plant material using onion layers as a biological target, and silver nanoaggregates and silver island films as enhancing plasmonic structures. Surface‐enhanced Raman scattering (SERS) enhancement allows the detection of strong Raman signatures of chemical constituents of the surface of the onion layer such as cellulose, proteins, and flavonols. Because of long‐time incubation, SERS sensors can access the extracellular space in the inner of the layer. The location of silver nanoparticles inside the onion layer has been monitored by the SERS images collected from chemicals present in the onion and/or reporter molecules attached to the nanoparticles. Our studies show a competitive adsorption of intrinsic bio molecules of the onion layer and reporter molecules. Different spectra from different places of the layer indicate the complex heterogeneous chemical structure of the plant material. The pH‐sensitive reporter molecule para mercapto benzoic acid attached to the nanoparticles allows us to infer pH values inside the extracellular matrix of the onion layer. Copyright © 2015 John Wiley & Sons, Ltd. Abstract : We report surface‐enhanced Raman scattering (SERS) on intact plant material such as onion layers using silver nanoaggregates and rough silver films as plasmonic nanostructures. SERS sensors can access the extracellular matrix of the onion and identify intrinsic biological molecules as well as infer the pH value.