Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig. Issue 1 (29th December 2015)

• Record Type: Journal Article
• Authors: Song, Yuning; Lai, Liangxue; Li, Li; Huang, Yongye; Wang, Anfeng; Tang, Xiaochun; Pang, Daxin; Li, Zhanjun; Ouyang, Hongsheng
• Is Part Of: FEBS open bio. Volume 6:Issue 1(2016)
• Page Start: 50
• Page End: 55
• Publication Date: 2015-12-29
• Subjects: Cre–loxP system -- pig -- SCNT -- transgene -- VASA; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.12005
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : The Cre– lox P system is a powerful tool for genetic analysis of distinct cell lineages and tissue‐specific gene knockout in animal models. VASA is specifically expressed in reproductive tissues, and is known to play important roles in spermatogenesis and germ‐cell growth. In this study, Cre recombinase transgenic pigs under the control of the VASA promoter were generated by somatic cell nuclear transfer. Germ cell‐specific expression of Cre recombinase in VASA ‐Cre transgenic pigs was shown by western blotting and immunohistochemistry. VASA ‐Cre transgenic pigs will be a useful tool for germ cell‐specific gene knockout and a disease model for disorders of the reproductive system.