Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity. Issue 3 (2nd December 2015)

• Record Type: Journal Article
• Authors: Blaeser, Andreas; Duarte Campos, Daniela Filipa; Puster, Uta; Richtering, Walter; Stevens, Molly M.; Fischer, Horst
• Is Part Of: Advanced healthcare materials. Volume 5:Issue 3(2016)
• Page Start: 326
• Page End: 333
• Publication Date: 2015-12-02
• Subjects: bioprinting -- hydrogels -- rheology -- shear stress -- stem cells; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201500677
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : A microvalve‐based bioprinting system for the manufacturing of high‐resolution, multimaterial 3D‐structures is reported. Applying a straightforward fluid‐dynamics model, the shear stress at the nozzle site can precisely be controlled. Using this system, a broad study on how cell viability and proliferation potential are affected by different levels of shear stress is conducted. Complex, multimaterial 3D structures are printed with high resolution. This work pioneers the investigation of shear stress‐induced cell damage in 3D bioprinting and might help to comprehend and improve the outcome of cell‐printing studies in the future.