A Universal Polymerase Chain Reaction Developer. (16th December 2015)
- Record Type:
- Journal Article
- Title:
- A Universal Polymerase Chain Reaction Developer. (16th December 2015)
- Main Title:
- A Universal Polymerase Chain Reaction Developer
- Authors:
- Valentini, Paola
Pompa, Pier Paolo - Abstract:
- Abstract: The versatility of PCR, the gold standard for amplification of DNA targets, is hampered by the laborious, multi‐step detection based on gel electrophoresis. We propose a one‐step, one‐tube method for the rapid (5 min) naked‐eye detection of PCR products, based on controlled aggregation of gold nanoparticles. Our method is universal, instrument‐free, and ultra‐sensitive, as it could detect as low as 0.01 zeptomoles of HIV template DNA in an excess of interfering human genomic DNA.
- Is Part Of:
- Angewandte Chemie. Volume 128:Number 6(2016)
- Journal:
- Angewandte Chemie
- Issue:
- Volume 128:Number 6(2016)
- Issue Display:
- Volume 128, Issue 6 (2016)
- Year:
- 2016
- Volume:
- 128
- Issue:
- 6
- Issue Sort Value:
- 2016-0128-0006-0000
- Page Start:
- 2197
- Page End:
- 2200
- Publication Date:
- 2015-12-16
- Subjects:
- DNA-Detektion -- Gold-Nanopartikel -- Kolorimetrische Detektion -- Polymerasekettenreaktion
Chemistry -- Periodicals
540 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1002/ange.201511010 ↗
- Languages:
- English
- ISSNs:
- 0044-8249
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 0902.000000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 30.xml