Nano-mole scale sequential signal assignment by 1H-detected protein solid-state NMR. Issue 81 (28th August 2015)

• Record Type: Journal Article
• Authors: Wang, Songlin; Parthasarathy, Sudhakar; Xiao, Yiling; Nishiyama, Yusuke; Long, Fei; Matsuda, Isamu; Endo, Yuki; Nemoto, Takahiro; Yamauchi, Kazuo; Asakura, Tetsuo; Takeda, Mitsuhiro; Terauchi, Tsutomu; Kainosho, Masatsune; Ishii, Yoshitaka
• Is Part Of: Chemical communications. Volume 51:Issue 81(2015)
• Page Start: 15055
• Page End: 15058
• Publication Date: 2015-08-28
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc04618a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We present a 3D 1 H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10–100 nmol of fully protonated proteins. Abstract : We present a 3D 1 H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10–100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13 C-detected SSNMR approach.