Sensitive detection of copper(ii) ions based on the conformational change of peptides by surface plasmon resonance spectroscopy. Issue 20 (28th September 2015)

• Record Type: Journal Article
• Authors: Chen, Hongxia; Jia, Shengsong; Zhang, Jiangjiang; Jang, Minji; Chen, Xiuying; Koh, Kwangnak; Wang, Zhaoxia
• Is Part Of: Analytical methods. Volume 7:Issue 20(2015)
• Page Start: 8942
• Page End: 8946
• Publication Date: 2015-09-28
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay02047f
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A novel quantitative copper (ii ) ion sensor is developed based on the conformational change of Cu 2+ binding peptides using surface plasmon resonance (SPR) spectroscopy. Abstract : Due to the significant role of copper(ii ) ions in many biochemical and physiological processes, sensitive detection of copper(ii ) ions has attracted great attention. A novel quantitative copper(ii ) ion sensor is developed based on the conformational change of Cu 2+ binding peptides using surface plasmon resonance (SPR) spectroscopy. The specific interaction between carboxyl groups of the peptide and Cu 2+ induces a conformational change from α-helix to β-sheet with decreasing hydrophilicity. The formation of Cu 2+ –peptide aggregates on the chip surface leads to a refractive index change, which results in a sensitive SPR signal change. Thus, Cu 2+ can be measured selectively and sensitively due to the conformational change of Cu 2+ specific binding peptides. With this well-designed sensing platform, the detection range of copper(ii ) ions is found to be 1 × 10 −12 M to 1 × 10 −6 M with a detection limit of 0.44 pM, which is 3 orders of magnitude lower than that in previous reports. The copper(ii ) ion sensor designed in this study is proposed for application in biological and environmental analysis.