Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots. Issue 20 (22nd July 2015)

• Record Type: Journal Article
• Authors: Griffiths, Rian L.; Dexter, Alex; Creese, Andrew J.; Cooper, Helen J.
• Is Part Of: Analyst. Volume 140:Issue 20(2015)
• Page Start: 6879
• Page End: 6885
• Publication Date: 2015-07-22
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an00933b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots. Abstract : Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.