Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide. Issue 21 (24th September 2015)

• Record Type: Journal Article
• Authors: Liu, Tianbao; Lin, Jie; Li, Zhen; Lin, Lin; Shen, Yuning; Zhu, Hailiang; Qian, Yong
• Is Part Of: Analyst. Volume 140:Issue 21(2015)
• Page Start: 7165
• Page End: 7169
• Publication Date: 2015-09-24
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an00119f
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive imaging of H2 S in living cells and living zebrafish in vivo . Abstract : We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio ( I 637 / I 558 ) ( R 2 = 0.9867) versus hydrogen sulfide concentration in the range of 1–12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2 S under in vitro physiological conditions and for imaging H2 S in living cells and living zebrafish in vivo .