Determination of Benzodiazepines in Beef by Magnetic Solid Phase Extraction and High-Performance Liquid Chromatography–Tandem Mass Spectrometry. (3rd March 2016)

• Record Type: Journal Article
• Authors: Kuang, Huijuan; Gan, Bei; Guo, Liang; Aguilar, Zoraida P.; Xu, Hengyi
• Is Part Of: Analytical letters. Volume 49:Number 4(2016)
• Page Start: 499
• Page End: 510
• Publication Date: 2016-03-03
• Subjects: Beef -- benzodiazepines -- high-performance liquid chromatography–tandem mass spectrometry -- magnetic solid-phase extraction; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2015.1076830
• Languages: English
• ISSNs: 0003-2719

Abstract

ABSTRACT: Sulfo-functionalized iron oxide magnetic nanobeads were employed as an adsorbent for magnetic solid-phase extraction of benzodiazepines in beef. The analytes were determined by high performance liquid chromatography–mass spectrometry. The sulfo-functionalized iron oxide magnetic nanobeads were characterized by transmission electron microscopy and infrared spectroscopy. The optimal pH, ionic strength, volume of sulfo-functionalized iron oxide magnetic nanobeads, and elution solvent were investigated for the isolation procedure. Under the optimized conditions, the limits of detection for diazepam, oxzepam, triazolam, and chlordiazepoxide were 0.79, 0.22, 0.13, and 0.23 ng/kg, respectively. The method was employed for the determination of the analytes in beef. Compared with established methods, magnetic solid phase extraction and high-performance liquid chromatography–tandem mass spectrometry method exhibited high sensitivity, rapid separation, and low cost.