Development of sandwich ELISA for quantification of the orange allergen profilin (Cit s 2). Issue 1 (2nd January 2016)

• Record Type: Journal Article
• Authors: Kiyota, Kyohei; Kawatsu, Kentaro; Sakata, Junko; Yoshimitsu, Masato; Akutsu, Kazuhiko; Kajimura, Keiji
• Is Part Of: Food and agricultural immunology. Volume 27:Issue 1(2016)
• Page Start: 128
• Page End: 137
• Publication Date: 2016-01-02
• Subjects: Citrus sinensis -- food allergy -- orange allergen -- profilin -- sandwich ELISA; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2015.1079599
• Languages: English
• ISSNs: 0954-0105

Abstract

Abstract : Oranges ( Citrus sinensis ) can provoke an allergic reaction in some individuals. The concentration of profilin (Cit s 2) – the major allergen in oranges – is unknown, but this knowledge could be useful for preventing the development of allergies. The aim of this study was to quantify the amount of Cit s 2 in Navel oranges and in different varieties of citrus fruit. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed using both monoclonal and polyclonal antibodies. Extracts of Navel oranges and 12 citrus fruits were tested using this ELISA. Cit s 2 levels in the Navel orange pulp and peel were 1.81 ± 0.74 and 1.19 ± 0.87 µg/g, respectively, with accumulation detected primarily in the pulp. These findings may help in the production of low-risk processed oranges for orange allergy sufferers through selection of orange varieties and parts with low profilin content during food processing.