Ternary Dinuclear Copper(II) Complexes of a Reduced Schiff Base Ligand with Diimine Coligands: DNA Binding, Cytotoxic Cell Apoptosis, and Apoptotic Mechanism. (20th November 2015)

• Record Type: Journal Article
• Authors: Yu, Hao; Yang, Yong; Li, Qiaoyu; Ma, Tieliang; Xu, Jun; Zhu, Taofeng; Xie, Jing; Zhu, Wenjiao; Cao, Zhihong; Dong, Kun; Huang, Jiancui; Jia, Lei
• Is Part Of: Chemical biology & drug design. Volume 87:Number 3(2016)
• Page Start: 398
• Page End: 408
• Publication Date: 2015-11-20
• Subjects: cell apoptosis -- cytotoxicity -- DNA‐binding properties -- ternary Cu(II) complexes; Drugs -- Design -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; 615.19005
• DOI: 10.1111/cbdd.12669
• Languages: English
• ISSNs: 1747-0277

Abstract

Abstract : A serial of mixed‐ligand Cu(II) complexes of the type [Cu(phens)(H2 PDILeu)]H2 O(1 ‐4 ) containing phens as 2, 2′‐bipyridyl (bpy, 1 ), 1, 10‐phenanthroline (phen, 2 ), dipyrido[3, 2‐d:2′, 3′‐f]quinoxaline (dpq, 3 ), and dipyrido[3, 2‐a:2′, 3′‐c]phenazine (dppz, 4 ) have been isolated and characterized. The interaction of the complexes with calf‐thymus DNA has been explored by physical methods to propose modes of DNA binding of the complexes, which indicate that4 interacts with DNA more strongly than all of the other complexes through intercalation interaction. Furthermore, cell apoptosis was detected by AnnexinV/PI flow cytometry and TUNEL assay and by Western blotting to detect the protein expression of p53, Bax, and Bcl‐2. All the three copper complexes can effectively induce apoptosis of the three human tumor cells, which was accompanied with upregulation of the expression of p53 and Bax, while Bcl‐2 decreased. Abstract : A serial of mixed ligand Cu(II) complexes have been isolated and characterized. The interaction of the complexes with calf thymus DNA has been explored by using physical methods to propose modes of DNA binding of the complexes, which indicate that4 interacts with DNA more strongly than all of the other complexes through intercalation interaction.