Detection of benzo[a]pyrene‐induced immunotoxicity in orange spotted grouper (Epinephelus coioides). Issue 3 (29th September 2014)

• Record Type: Journal Article
• Authors: Khaniyan, Maryam; Salamat, Negin; Safahieh, Alireza; Movahedinia, Abdolali
• Is Part Of: Environmental toxicology. Volume 31:Issue 3(2016)
• Page Start: 329
• Page End: 338
• Publication Date: 2014-09-29
• Subjects: polycyclic aromatic hydrocarbon -- innate immunity -- acquired immunity -- orange spotted grouper; Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.22047
• Languages: English
• ISSNs: 1520-4081

Abstract

ABSTRACT: This study aimed to investigate the effects of benzo[ a ]pyrene (BaP) on immune status of orange spotted grouper ( Epinephelus coioides ). Fish were injected with 2, 20 and 35 mg/kg‐bw of BaP and were kept under laboratory conditions for 14 days. Blood samples were taken at days 1, 4, 7, and 14 and changes in total WBC and RBC, phagocytosis, lysozyme activity, lysosomal membrane stability, immunoglobulin M (IgM) level and antibacterial activity were evaluated. Also BaP bioaccumulation in fish muscle was measured. BaP concentration in the muscle of treated fish reached a maximum level after 4 days ( P < 0.05). Exposure of fish to BaP resulted in a significant decrease of total RBC and WBC, lysozyme activity, lysosomal membrane stability, IgM level and antibacterial activity after 4 days and phagocytosis after 7 days of the experiment ( P < 0.05). Totally, the results revealed BaP ability to suppress the fish immune function. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 329–338, 2016.