Spontaneous initiation, promotion and progression of colorectal cancer in the novel A/J Min/+ mouse. Issue 8 (28th November 2015)

• Record Type: Journal Article
• Authors: Sødring, Marianne; Gunnes, Gjermund; Paulsen, Jan Erik
• Is Part Of: International journal of cancer. Volume 138:Issue 8(2016:Apr. 15)
• Page Start: 1936
• Page End: 1946
• Publication Date: 2015-11-28
• Subjects: colorectal cancer -- A/J Min/+ mouse -- intestinal tumorigenesis -- carcinoma; Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.29928
• Languages: English
• ISSNs: 0020-7136

Abstract

Abstract : The C57BL/6J multiple intestinal neoplasia (Min/+) mouse is a widely used murine model for familial adenomatous polyposis, a hereditary form of human colorectal cancer. However, it is a questionable model partly because the vast majority of tumors arise in the small intestine, and partly because the fraction of tumors that progress to invasive carcinomas is minuscule. A/J mice are typically more susceptible to carcinogen‐induced colorectal cancer than C57BL/6J mice. To investigate whether the novel Min/+ mouse on the A/J genetic background could be a better model for colorectal cancer, we examined the spontaneous intestinal tumorigenesis in 81 A/J Min/+ mice ranging in age from 4 to 60 weeks. The A/J Min/+ mouse exhibited a dramatic increase in number of colonic lesions when compared to what has been reported for the conventional Min/+ mouse; however, an increase in small intestinal lesions did not occur. In addition, this novel mouse model displayed a continual development of colonic lesions highlighted by the transition from early lesions (flat ACF) to tumors over time. In mice older than 40 weeks, 13 colonic (95% CI: 8.7–16.3) and 21 small intestinal (95% CI: 18.6‐24.3) tumors were recorded. Notably, a considerable proportion of those lesions progressed to carcinomas in both the colon (21%) and small intestine (51%). These findings more closely reflect aspects of human colorectal carcinogenesis. In conclusion, the novel A/J Min/+ mouse may be a relevant model for initiation, promotion and progression of colorectal cancer. Abstract : What's new? Human colorectal cancer (CRC) manifests itself as benign tumors in the colon that progress to carcinomas. In contrast, most rodent CRC models for Apc‐driven tumorigenesis primarily develop benign tumors in the small intestine instead of the colon, which rarely progress to carcinomas. Here, the novel A/J Min/+ mouse was observed to spontaneously develop numerous colonic lesions, and a progression from benign tumor to carcinoma was found in both the colon and small intestine.