Asymmetric Anion‐selective Membrane Electrode for Determining Nitric Oxide Release Rates from Ppolymeric Films/Electrochemical Devices. Issue 2 (29th July 2015)

• Record Type: Journal Article
• Authors: Zajda, Joanna; Crist, Natalie R.; Malinowska, Elżbieta; Meyerhoff, Mark E.
• Is Part Of: Electroanalysis. Volume 28:Issue 2(2016)
• Page Start: 277
• Page End: 281
• Publication Date: 2015-07-29
• Subjects: Ion‐selective electrodes -- Nitric oxide -- Oxyhemoglobin; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500395
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A novel potentiometric method for the determination of nitric oxide (NO) is presented. The assessment of NO levels is based on the rapid reaction of NO with oxyhemoglobin, during which nitrate is formed and then detected potentiometrically using a polymeric membrane anion‐selective electrode. The composition of the anion‐selective membrane is optimized to eliminate the response to high levels of oxyhemoglobin reagent used to react with NO to form NO3 − . The practical utility of the method is demonstrated by determining the NO release rates from NO donor‐doped polymeric films, as well as from a new electrochemical NO generation system within the range of 10 to 55×10 −10 mol ⋅ min −1 . The values measured with the new assay system are shown to correlate well with a chemiluminescence reference method after accounting for the trapping efficiency of NO by the oxyhemoglobin solution. Abstract :