A Novel Method of High Sensitive Determination of Prednisolone on Renewable Mercury Film Silver Based Electrode. Issue 2 (5th August 2015)

• Record Type: Journal Article
• Authors: Smajdor, Joanna; Piech, Robert; Paczosa‐Bator, Beata
• Is Part Of: Electroanalysis. Volume 28:Issue 2(2016)
• Page Start: 394
• Page End: 400
• Publication Date: 2015-08-05
• Subjects: Prednisolone -- voltammetry -- mercury film electrode -- steroid determination; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500262
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Fast and sensitive method of prednisolone determination at renewable mercury film electrode using differential pulse adsorptive stripping voltammetry (DPAdSV) was successfully developed. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition were optimized. A linear voltammetric response for analyte was obtained in the concentration range from 0.05 µmol L −1 (18 µg L −1 ) to 2.25 µmol L −1 (0.81 mg L −1 ), with a detection limit of 0.01 µmol L −1 for preconcentration time of 20 s. Repeatability of method was determined as RSD % for prednisolone concentration of 0.04 µmol L −1 as 1.6 % (n=9). The proposed method was successfully applied and validated by recovery parameter of prednisolone in simulated sample and pharmaceutical product. Abstract :