Controllable Switching of Enzyme Activity by Poly(N‐isopropylacrylamide)‐Based Microgels Through Mineralization of Calcium Carbonate in High Pressure CO2. Issue 2 (12th December 2015)

• Record Type: Journal Article
• Authors: Yang, Xiaoxi; Zhang, Xiaogang; Shang, Wenting; Zhang, Shujuan
• Is Part Of: Clean. Volume 44:Issue 2(2016:Feb.)
• Page Start: 189
• Page End: 194
• Publication Date: 2015-12-12
• Subjects: Alginate -- Enzymatic hydrolysis -- Stimuli‐responsive hydrogels -- Supercritical carbon dioxide -- Thermally responsive materials; Water quality -- Periodicals; Water -- Pollution -- Periodicals; Pollution -- Periodicals; Bioremediation -- Periodicals; Sewage -- Periodicals; Water chemistry -- Periodicals; 333.7205
• DOI: 10.1002/clen.201300967
• Languages: English
• ISSNs: 1863-0650

Abstract

Abstract : Stimuli‐responsive hydrogels with high strength and toughness have received great interest in recent years. In this study, temperature‐responsive composite hydrogels involved the Candida antarctica lipase B were prepared by using sodium alginate and poly( N ‐isopropylacrylamide), and were evaluated as catalysts using the model reaction of hydrolysis of p ‐nitrophenol butyrate. The experimental results demonstrated that enzymatic catalyst immobilized in the thermoresponsive carriers offered possibilities for external switching and manipulation for objective reaction system. The mechanical properties of the enzyme–polymer composites pretreated by high pressure carbon dioxide could be significantly improved. The number of recycling use was significantly increased. This work provided a new approach to smart regulation for enzymatic reaction. Abstract : The enzymatic catalyst immobilized in thermoresponsive carriers offered possibilities for external switching and manipulation for the objective reaction system. Blocking the active sites of the enzyme due to a change of the crosslinking density with temperature in the smart polymer plays an important role in the improved on and off reversibility of the enzyme switch.