Double stranded promoter region of BRAF undergoes to structural rearrangement in nearly physiological conditions. Issue 16 (2nd July 2015)

• Record Type: Journal Article
• Authors: Greco, Maria Laura; Folini, Marco; Sissi, Claudia
• Is Part Of: FEBS letters. Volume 589:Issue 16(2015)
• Page Start: 2117
• Page End: 2123
• Publication Date: 2015-07-02
• Subjects: BRAF -- G-quadruplex -- i-motif -- Oncogene; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.06.025
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The folding of oncogene promoters into non‐canonical DNA secondary structures is considered a strategy to control gene expression. Herein, we focused on a 30 bases sequence located upstream of the transcription start site of BRAF (Braf‐176) that contains 80% of guanines. We analyzed the structural behavior of the G‐ and C‐rich strands. By the use of spectroscopic and electrophoretic techniques we confirmed that they actually fold into a predominant antiparallel G‐quadruplex and into an i‐motif, respectively, and that they can coexist at nearly physiological conditions. Finally, the influence of several factors (KCl, pH, PEG200 ) on the conversion of the double stranded form of the oncogene promoter into the two above mentioned non‐canonical structures has been explored. Abstract : The G‐rich strand of Braf‐176 folds into a predominant antiparallel G‐quadruplex. The C‐rich strand of Braf‐176 assumes an i‐motif conserved up to pH 7.0. The double stranded form of Braf‐176 is highly stable. KCl, PEG and pH modulate the formation of G4 and iM from the ds_Braf‐176.