Membrane protein production in Escherichia coli cell‐free lysates. Issue 15 (1st May 2015)

• Record Type: Journal Article
• Authors: Henrich, Erik; Hein, Christopher; Dötsch, Volker; Bernhard, Frank
• Is Part Of: FEBS letters. Volume 589:Issue 15(2015)
• Page Start: 1713
• Page End: 1722
• Publication Date: 2015-05-01
• Subjects: Synthetic biology -- Nanodiscs -- Membrane protein solubilization -- Expression modes -- Escherichia coli lysates; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.04.045
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Cell‐free protein production has become a core technology in the rapidly spreading field of synthetic biology. In particular the synthesis of membrane proteins, highly problematic proteins in conventional cellular production systems, is an ideal application for cell‐free expression. A large variety of artificial as well as natural environments for the optimal co‐translational folding and stabilization of membrane proteins can rationally be designed. The high success rate of cell‐free membrane protein production allows to focus on individually selected targets and to modulate their functional and structural properties with appropriate supplements. The efficiency and robustness of lysates from Escherichia coli strains allow a wide diversity of applications and we summarize current strategies for the successful production of high quality membrane protein samples.