Acid‐sensing ion channel 1a is involved in acid‐induced osteoclastogenesis by regulating activation of the transcription factor NFATc1. Issue 19 (28th August 2013)

• Record Type: Journal Article
• Authors: Li, Xia; Xu, Rui-Sheng; Jiang, Dong-Lin; He, Xin-Long; Jin, Chen; Lu, Wei-Guo; Su, Qiang; Yuan, Feng-Lai
• Is Part Of: FEBS letters. Volume 587:Issue 19(2013)
• Page Start: 3236
• Page End: 3242
• Publication Date: 2013-08-28
• Subjects: Osteoclast -- Acid-sensing ion channel -- Acidosis -- Osteoclastogenesis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.08.017
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : It has been known that osteoclastogenesis is induced by extracellular acidosis‐evoked the rise of intracellular calcium ([Ca 2+ ]i), which regulate activation of the transcription factor nuclear factor of activated T cells c1 (NFATc1). However, the acid‐sensing ion channels (ASICs) involved remain largely unknown. Here, we show that ASIC1a, ASIC1b, ASIC2a, and ASIC3 are expressed in rat osteoclasts, and only ASIC1a is highly upregulated in response to acidosis. Both the ASIC1a‐specific blocker PcTX1 and specific siRNA significantly reduce this increase in acid‐induced [Ca 2+ ]i and acid‐induced nuclear translocation of NFATc1, and inhibit acid‐induced osteoclast differentiation and bone resorption. These findings show that ASIC1a‐mediated calcium entry plays a critical role in osteoclastogenesis by regulating activation of the NFATc1. Abstract : ASIC1a is expressed in rat osteoclasts and highly upregulated in response to acidosis. ASIC1a mediates acid‐induced increases in intracellular calcium [Ca 2+ ]i in osteoclasts. Acid‐induced [Ca 2+ ]i increase via ASIC1a is responsible for osteoclastogenesis. ASIC1 is involved in acid‐stimulated NFATc1 signaling in osteoclastogenesis.