Insulin receptor substrate‐1 (IRS‐1) forms a ribonucleoprotein complex associated with polysomes. Issue 15 (13th June 2013)

• Record Type: Journal Article
• Authors: Ozoe, Atsufumi; Sone, Meri; Fukushima, Toshiaki; Kataoka, Naoyuki; Arai, Toshiya; Chida, Kazuhiro; Asano, Tomoichiro; Hakuno, Fumihiko; Takahashi, Shin-Ichiro
• Is Part Of: FEBS letters. Volume 587:Issue 15(2013)
• Page Start: 2319
• Page End: 2324
• Publication Date: 2013-06-13
• Subjects: IRSs -- insulin receptor substrates -- IGFs -- insulin-like growth factors -- PABPC1 -- poly(A) binding protein cytoplasmic 1 -- MALDI-TOF-MS -- matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry -- BN-PAGE -- blue native-polyacrylamide gel electrophoresis -- mRNA -- messenger RNA -- mRNP -- messenger ribonucleoprotein -- eIF -- eukaryotic initiation factor -- PI3K -- phosphatidylinositol 3-kinase -- MAPK -- mitogen-activated protein kinase -- mTOR -- mammalian target of rapamycin -- EJC -- exon junction complex -- 4EBPs -- 4E binding proteins -- rpS6 -- ribosomal subunit protein S6 -- S6Ks -- S6 protein kinases -- PIC -- translation preinitiation complex -- HEK293 -- human embryonic kidney 293 -- DMEM -- Dulbecco's modified Eagle's medium -- RNase A -- ribonuclease A -- FBS -- fetal bovine serum -- Insulin-like growth factor-I (IGF-I) -- Insulin receptor substrate -- RNA -- Translation; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.05.066
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Insulin receptor substrates (IRSs) are known to play important roles in mediating intracellular insulin‐like growth factors (IGFs)/insulin signaling. In this study, we identified components of messenger ribonucleoprotein (mRNP) as IRS‐1‐associated proteins. IRS‐1 complex formation analysis revealed that IRS‐1 is incorporated into the complexes of molecular mass more than 1000 kDa, which were disrupted by treatment with RNase. Furthermore, oligo(dT) beads precipitated IRS‐1 from cell lysates, showing that the IRS‐1 complexes contained messenger RNA. Taken together with the data that IRS‐1 was fractionated into the polysome‐containing high‐density fractions, we concluded that IRS‐1 forms the novel complexes with mRNPs. Abstract : IRS‐1 forms complexes with messenger RNA. IRS‐1 interacts with PABPC1, eIF4E and eIF4G in RNA‐dependent manner. IRS‐1 is distributed into high‐density fractions containing polysomes in proliferating cells.