RNA self‐processing: Formation of cyclic species and concatemers from a small engineered RNA. Issue 15 (22nd June 2013)

• Record Type: Journal Article
• Authors: Petkovic, Sonja; Müller, Sabine
• Is Part Of: FEBS letters. Volume 587:Issue 15(2013)
• Page Start: 2435
• Page End: 2440
• Publication Date: 2013-06-22
• Subjects: Cleavage -- Ligation -- Nucleic acid -- Ribozyme -- RNA -- Self-processing; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.06.013
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : We have engineered a self‐processing RNA, derived from the hairpin ribozyme that runs through a cascade of cleavage and ligation reactions thereby changing its topology. The first two cleavage events leave the resulting RNA with a 5′‐OH group and a 2′, 3′‐cyclic phosphate. Thus, upon refolding, intramolecular ligation delivers a cyclic species. In addition, we demonstrate formation of concatemers resulting from multiple intermolecular ligations. Our results demonstrate the potential of RNA for self‐supported topology changes and support the suggestion of 2′, 3′‐cyclic phosphates being suitable activated building blocks for reversible phosphodiester bond formation in the RNA world. Abstract : Engineered hairpin ribozyme constructs self‐process forming a variety of products. Ribozyme supported cyclization competes with concatemerization. Cyclic species can be reliably detected by 2D gel electrophoresis.