HIV‐1 envelope trimer has similar binding characteristics for carbohydrate‐binding agents as monomeric gp120. Issue 7 (26th February 2013)

• Record Type: Journal Article
• Authors: Hoorelbeke, Bart; van Montfort, Thijs; Xue, Jie; LiWang, Patricia J.; Tanaka, Haruo; Igarashi, Yasuhiro; Van Damme, Els J.M.; Sanders, Rogier W.; Balzarini, Jan
• Is Part Of: FEBS letters. Volume 587:Issue 7(2013)
• Page Start: 860
• Page End: 866
• Publication Date: 2013-02-26
• Subjects: AH -- actinohivin -- GRFT -- griffithsin -- PRM-S -- pradimicin S -- SPR -- surface plasmon resonance -- HIV -- human immunodeficiency virus -- CBA -- carbohydrate-binding agent -- HHA -- Hippeastrum hybrid agglutinin -- UDA -- Urtica dioica agglutinin -- DC-SIGN -- dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3) grabbing non-integrin -- Carbohydrate-binding agent -- High-mannose N-glycan -- Monomeric gp120 -- Surface plasmon resonance -- Trimeric gp140; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.02.037
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : gp120 binds to AH by surface plasmon resonance (View Interaction: 1, 2) Abstract : ► Similar binding kinetics of CBAs for monomeric gp120 and trimeric gp140. ► Similar binding kinetics of the Ab 2G12 and sCD4 for monomeric gp120 and trimeric gp140. ► Two‐ to threefold stronger affinity of Ab b12 and DC‐SIGN for trimeric gp140. ► SPR‐directed binding kinetics with monomeric gp120 are relevant for native gp140 trimer.