Crystal structure of Ruminococcus albus cellobiose 2‐epimerase: Structural insights into epimerization of unmodified sugar. Issue 7 (24th February 2013)

• Record Type: Journal Article
• Authors: Fujiwara, Takaaki; Saburi, Wataru; Inoue, Sota; Mori, Haruhide; Matsui, Hirokazu; Tanaka, Isao; Yao, Min
• Is Part Of: FEBS letters. Volume 587:Issue 7(2013)
• Page Start: 840
• Page End: 846
• Publication Date: 2013-02-24
• Subjects: CE -- cellobiose 2-epimerase -- RaCE -- Ruminococcus albus CE -- EcCE -- Eubacterial cellulosolvens CE -- BfCE -- Bacteroides fragilis CE -- RmCE -- Rhodothermus marinas CE -- AGE -- N-acetyl-d-glucosamine 2-epimerase -- pAGE -- porcine kidney AGE -- aAGE -- Anabaena sp. CH1 AGE -- GlcNAc -- N-acetyl-d-glucosamine -- ManNAc -- N-acetyl-d-mannosamine -- EcYihS -- Escherichia coli YihS -- SeYihS -- Salmonella enterica YihS -- PLP -- pyridoxal phosphate -- X-ray crystallography -- Cellobiose 2-epimerase -- Deprotonation -- Unmodified sugar -- Structure; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.02.007
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : RaCE and X‐ray crystallography (View interaction) Abstract : ► We determined the crystal structure of Ra CE. ► We found that His184 is required for Ra CE activity. ► We proposed a new catalytic mechanism for CE involving three histidines.