Interactions between subunits a and b in the rotary ATP synthase as determined by cross‐linking. Issue 7 (14th February 2013)

• Record Type: Journal Article
• Authors: DeLeon-Rangel, Jessica; Ishmukhametov, Robert R.; Jiang, Warren; Fillingame, Robert H.; Vik, Steven B.
• Is Part Of: FEBS letters. Volume 587:Issue 7(2013)
• Page Start: 892
• Page End: 897
• Publication Date: 2013-02-14
• Subjects: TM -- transmembrane -- a_W111 -- residue W111 of subunit a -- BPM -- benzophenone-4-maleimide -- HA -- hemagglutinin -- ATP synthase -- Transmembrane helix -- Disulfide cross-linking -- Benzophenone-4-maleimide -- Subunit a -- Subunit b; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.02.012
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The interaction of the membrane traversing stator subunits a and b of the rotary ATP synthase was probed by substitution of a single Cys into each subunit with subsequent Cu 2+ catalyzed cross‐linking. Extensive interaction between the transmembrane (TM) region of one b subunit and TM2 of subunit a was indicated by cross‐linking with 6 Cys pairs introduced into these regions. Additional disulfide cross‐linking was observed between the N‐terminus of subunit b and the periplasmic loop connecting TM4 and TM5 of subunit a . Finally, benzophenone‐4‐maleimide derivatized Cys in the 2–3 periplasmic loop of subunit a were shown to cross‐link with the periplasmic N‐terminal region of subunit b . These experiments help to define the juxtaposition of subunits b and a in the ATP synthase. Abstract : ► Interactions of subunits a and b of the rotary ATP synthase were examined. ► Cys substitutions were used for disulfide formation or with a bi‐functional reagent. ► Cross‐linked products indicated that one b subunit has contact with TM2 of subunit a . ► Additional cross‐links were detected between subunits a and b and between b and c . ► A model is presented in which one b subunit is near a TM2 and the other is near a TM5.