CIZ1, a p21Cip1/Waf1‐interacting protein, functions as a tumor suppressor in vivo. Issue 10 (11th April 2013)

• Record Type: Journal Article
• Authors: Nishibe, Rio; Watanabe, Wataru; Ueda, Takeshi; Yamasaki, Norimasa; Koller, Richard; Wolff, Linda; Honda, Zen-ichiro; Ohtsubo, Motoaki; Honda, Hiroaki
• Is Part Of: FEBS letters. Volume 587:Issue 10(2013)
• Page Start: 1529
• Page End: 1535
• Publication Date: 2013-04-11
• Subjects: CIZ1 (Cip1 interacting zinc finger protein 1) -- p21Cip1/Waf1 -- Cell proliferation -- Tumorigenesis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.03.034
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : CIZ1 is a nuclear protein involved in DNA replication and is also implicated in human diseases including cancers. To gain an insight into its function in vivo, we generated mice lacking Ciz1 . Ciz1 ‐deficient ( Ciz1 −/− ) mice grew without any obvious abnormalities, and Ciz1 −/− mouse embryonic fibroblasts (MEFs) did not show any defects in cell cycle status, cell growth, and DNA damage response. However, Ciz1 −/− MEFs were sensitive to hydroxyurea‐mediated replication stress and susceptible to oncogene‐induced cellular transformation. In addition, Ciz1 −/− mice developed various types of leukemias by retroviral insertional mutagenesis. These results indicate that CIZ1 functions as a tumor suppressor in vivo. Abstract : Ciz1 ‐deficient ( Ciz1 −/− ) mouse embryonic fibroblasts (MEFs) did not show defects in cell cycle status, cell growth, and DNA damage response. Ciz1 −/− MEFs were sensitive to replication stress and susceptible to cellular transformation. Ciz1 −/− mice developed leukemias by retroviral insertional mutagenesis. CIZ1 functions as a tumor suppressor in vivo.