Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X‐ray crystallography. Issue 7 (19th February 2013)

• Record Type: Journal Article
• Authors: Nkansah, Edwin; Shah, Rahi; Collie, Gavin W.; Parkinson, Gary N.; Palmer, Jonathan; Rahman, Khondaker M.; Bui, Tam T.; Drake, Alex F.; Husby, Jarmila; Neidle, Stephen; Zinzalla, Giovanna; Thurston, David E.; Wilderspin, Andrew F.
• Is Part Of: FEBS letters. Volume 587:Issue 7(2013)
• Page Start: 833
• Page End: 839
• Publication Date: 2013-02-19
• Subjects: STAT3 -- signal transducer and activator of transcription 3 -- SH2 -- src homology 2 domain -- STAT3βtc -- the STAT3 core consisting of amino acid residues 127–722 -- YFP -- yellow variant of eGFP, the enhanced green fluorescent protein -- Signal transducer and activator of transcription 3 -- STAT3 -- PEMSA -- M67 -- SH2 -- X-ray crystallography; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.01.065
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : pSTAT3βtc and pSTAT3βtc bind by molecular sieving (View interaction) Abstract : ► Stoichiometric uSTAT3 interaction with M67 DNA. ► Interaction is weaker compared to pSTAT3 M67 DNA interaction. ► Report of a novel protein electrophoretic mobility shift assay (PEMSA).