Full‐length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells. Issue 5 (21st January 2013)

• Record Type: Journal Article
• Authors: Ng, Yuen-Keng; Lau, Wing-Sze; Lui, Vivian Wai Yan; Cheng, Alfred Sze-Lok; Ng, Patrick Kwok-Shing; Tsui, Stephen Kwok-Wing; Cheung, Yue Sun; Lai, Paul Bo San
• Is Part Of: FEBS letters. Volume 587:Issue 5(2013)
• Page Start: 496
• Page End: 503
• Publication Date: 2013-01-21
• Subjects: HCC -- hepatocellular carcinoma -- Mst -- mammalian sterile 20-like kinase -- Stk -- sterile 20-like kinase -- PAK -- p21 activated kinase -- RASSF -- RAS Association domain family -- GAPDH -- glyceraldehydes-3-phosphate dehydrogenase -- PARP -- poly ADP-ribose polymerase -- FL -- full length form -- NT -- amino terminal cleaved form -- Stk4 -- NORE1B -- Hep3B -- Huh7 -- PLC/PRF/5 -- Akt -- Staurosporine; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.01.018
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full‐length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full‐length Mst1 expression increased the growth of HCC cells by 55–80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full‐length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation. Abstract : ► Full‐length Mst1 is predominantly expressed in HCC tumors. ► Transfection of full‐length Mst1 promotes human HCC cellular growth. ► The putative tumor suppressor NORE1B suppresses the Mst1growth promoting activity. ► Our findings suggest that Mst1 promotes HCC growth upon NORE1B downregulation.