Identification of minimum Rpn4‐responsive elements in genes related to proteasome functions. Issue 8 (3rd March 2015) - Shirozu, Ryohei
Abstract : The proteasome is an essential, 66‐subunit protease that mediates ubiquitin‐dependent proteolysis. The transcription factor Rpn4 regulates concerted expression of proteasome subunits to increase the proteasome by recognizing nonamer proteasome‐associated control element (PACE) elements on the promoter regions. However, the genes for proteasome assembly chaperones and some of the subunits have no PACEs. Here we identified a minimal hexamer "PACE‐core" sequence that responds to Rpn4. PACE‐cores are found in many genes related to proteasome function including the assembly chaperones, but cannot substitute for PACE of the subunits. Our results add a new layer of complexity in transcriptional regulation of genes involved in protein degradation. Abstract : Rpn4 recognizes not only the typical nonamer PACE but a hexamer PACE‐core sequence. Many genes involved in proteasome function have PACE‐cores and are induced by Rpn4. The finding of the PACE‐core expands the role of Rpn4 under proteasome impairment.