Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells. Issue 10 (30th March 2015)

• Record Type: Journal Article
• Authors: Okumura, Nobuaki; Ikeda, Masanori; Satoh, Shinya; Dansako, Hiromichi; Sugiyama, Masaya; Mizokami, Masashi; Kato, Nobuyuki
• Is Part Of: FEBS letters. Volume 589:Issue 10(2015)
• Page Start: 1112
• Page End: 1118
• Publication Date: 2015-03-30
• Subjects: Hepatitis B virus -- Hepatitis B virus replication -- FOXA1 -- FOXA2 -- FOXA3 -- HNF3; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.03.022
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Hepatitis B virus (HBV) replication is controlled by liver‐enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH‐7 treated with individual FOXA members‐specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA‐induced HuH‐7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication. Abstract : FOXA members negatively regulate HBV replication in human hepatoma cell lines. The suppression of pgRNA by FOXA2 is strongest in all FOXA members. The regulation of small S expression is different between FOXA1/2 and FOXA3. The regulation of nuclear hormone receptors is different among FOXA members.