An evolutionarily‐unique heterodimeric voltage‐gated cation channel found in aphids. Issue 5 (27th January 2015)

• Record Type: Journal Article
• Authors: Amey, Joanna S.; O'Reilly, Andrias O.; Burton, Mark J.; Puinean, Alin M.; Mellor, Ian R.; Duce, Ian R.; Field, Linda M.; Wallace, B.A.; Williamson, Martin S.; Davies, T.G. Emyr
• Is Part Of: FEBS letters. Volume 589:Issue 5(2015)
• Page Start: 598
• Page End: 607
• Publication Date: 2015-01-27
• Subjects: BLAST -- basic local alignment search tool -- bp -- base pair -- cDNA -- DNA complementary to RNA -- DDT -- dichlorodiphenyltrichloroethane -- kb -- kilobase(s) or 1000 base pairs -- MYA -- million years ago -- Nav -- voltage-gated sodium channel -- NCBI -- The National Center for Biotechnology Information -- PCR -- polymerase chain reaction -- RACE -- Rapid Amplification of cDNA ends -- RNA -- ribonucleic acid -- TM -- trans membrane -- TTX -- tetrodotoxin -- Voltage-gated sodium channel -- Aphid -- Evolution -- Tetrodotoxin; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.01.020
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : We describe the identification in aphids of a unique heterodimeric voltage‐gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. We demonstrate that this channel has most likely arisen by adaptation (gene fission or duplication) of an invertebrate ancestral mono(hetero)meric channel. This is the only identifiable voltage‐gated sodium channel homologue in the aphid genome(s), and the channel's novel selectivity filter motif (DENS instead of the usual DEKA found in other eukaryotes) may result in a loss of sodium selectivity, as indicated experimentally in mutagenised Drosophila channels. Abstract : Aphids have a unique heterodimeric voltage‐gated sodium channel. The aphid channel has an atypical ion‐selectivity filter (DENS rather than DEKA). The channel's novel selectivity filter may result in a loss of sodium selectivity. This is the only identifiable voltage‐gated sodium channel in aphid genome(s). This channel has most likely arisen by gene fission or gene duplication.