VCP/Cdc48 rescues the growth defect of a GPI10 mutant in yeast. Issue 5 (24th January 2015)

• Record Type: Journal Article
• Authors: Ohnuma, Yohei; Takata, Takahiro; Kawawaki, Junko; Yasuda, Kunihiko; Tanaka, Keiji; Kimura, Yoko; Kakizuka, Akira
• Is Part Of: FEBS letters. Volume 589:Issue 5(2015)
• Page Start: 576
• Page End: 580
• Publication Date: 2015-01-24
• Subjects: Cdc48 -- Endoplasmic reticulum -- Gpi10 -- VCP -- Temperature-sensitivity -- GPI-anchor; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2015.01.017
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : We identified a yeast mutant with temperature‐sensitive growth defects that were rescued by VCP expression. The mutation occurred in GPI10, which encodes a mannosyl transferase for glycosylphosphatidylinositol anchor formation in the endoplasmic reticulum, and caused a Gly469Glu substitution in Gpi10. The mutant exhibited increased unfolded protein response, which was partially rescued by VCP or Cdc48, and showed sensitivity against cell‐wall stressors, which were not rescued by VCP. These results suggest a potential link between VCP/Cdc48 and Gpi10 functions in the control of cell growth. Abstract : GPI10 encodes a mannosyl transferase for GPI‐anchor formation in the ER. VCP/Cdc48 expression rescued the temperature‐sensitive yeast mutant of GPI10 . The mutant showed sensitivity to cell‐wall stress and increased UPR response. VCP expression only partially rescued ER‐related defective phenotypes.