Distinct tRNA modifications in the thermo‐acidophilic archaeon, Thermoplasma acidophilum. Issue 21 (25th September 2013)

• Record Type: Journal Article
• Authors: Tomikawa, Chie; Ohira, Takayuki; Inoue, Yasushi; Kawamura, Takuya; Yamagishi, Akihiko; Suzuki, Tsutomu; Hori, Hiroyuki
• Is Part Of: FEBS letters. Volume 587:Issue 21(2013)
• Page Start: 3575
• Page End: 3580
• Publication Date: 2013-09-25
• Subjects: tRNA -- Archaea -- Archaeosine -- 7-Methylguanine -- 4-Thiouridine -- 5-Carbamoylmethyuridine; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.09.021
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Thermoplasma acidophilum is a thermo‐acidophilic archaeon. We purified tRNA Leu (UAG) from T. acidophilum using a solid‐phase DNA probe method and determined the RNA sequence after determining via nucleoside analysis and m 7 G‐specific aniline cleavage because it has been reported that T. acidophilum tRNA contains m 7 G, which is generally not found in archaeal tRNAs. RNA sequencing and liquid chromatography–mass spectrometry revealed that the m 7 G modification exists at a novel position 49. Furthermore, we found several distinct modifications, which have not previously been found in archaeal tRNA, such as 4‐thiouridine9, archaeosine13 and 5‐carbamoylmethyuridine34. The related tRNA modification enzymes and their genes are discussed. Abstract : Thermoplasma acidophilum tRNA Leu contains distinct modified nucleotides. 7‐Methylguanine was found at novel position 49 in tRNA Leu . Archaeosines were found at the novel position 13 as well as the common position 15. 4‐Thiouridine at position 9 was newly found in archaeal tRNA. 5‐Carbamoylmethyuridine at position 34 was newly found in archaeal tRNA.