DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1–Dio3 domain. Issue 24 (11th November 2014)

• Record Type: Journal Article
• Authors: Zeng, Tie-Bo; He, Hong-Juan; Han, Zheng-Bin; Zhang, Feng-Wei; Huang, Zhi-Jun; Liu, Qi; Cui, Wei; Wu, Qiong
• Is Part Of: FEBS letters. Volume 588:Issue 24(2014)
• Page Start: 4665
• Page End: 4671
• Publication Date: 2014-11-11
• Subjects: DNA methylation -- Genomic imprinting -- Differentially methylated region -- CCCTC-binding factor -- Dlk1–Dio3 domain; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.10.038
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The mouse delta‐like homolog 1 and type III iodothyronine deiodinase ( Dlk1 – Dio3 ) imprinted domain contains three known paternally methylated differentially methylated regions (DMRs): intergenic DMR (IG‐DMR), maternally expressed 3‐DMR ( Gtl2 ‐DMR), and Dlk1 ‐DMR. Here, we report the first maternally methylated DMR, CpG island 2 (CGI‐2), is located approximately 800 bp downstream of miR‐1188. CGI‐2 is highly methylated in sperm and oocytes, de‐methylated in pre‐implantation embryos, and differentially re‐methylated during post‐implantation development. CGI‐2, similarly to Gtl2 ‐DMR and Dlk1 ‐DMR, acquires differential methylation prior to embryonic day 7.5 (E7.5). Both H3K4me3 and H3K9me3 histone modifications are enriched at CGI‐2. Furthermore, CCCTC‐binding factor (CTCF) binds to both alleles of CGI‐2 in vivo . These results contribute to the investigation of imprinting regulation in this domain. Abstract : CGI‐2 is the first maternally methylated DMR found in the Dlk1 – Dio3 domain. CGI‐2 undergoes a complex demethylation and re‐methylation process in pre‐ and post‐implantation embryos. CGI‐2 acquires differential methylation at around the same time as Gtl2 ‐DMR and Dlk1 ‐DMR. CTCF binds to CGI‐2 in vivo .