MiR‐92a inhibits porcine ovarian granulosa cell apoptosis by targeting Smad7 gene. Issue 23 (27th October 2014)

• Record Type: Journal Article
• Authors: Liu, Jiying; Yao, Wang; Yao, Yong; Du, Xing; Zhou, Jilong; Ma, Baiquan; Liu, Honglin; Li, Qifa; Pan, Zengxiang
• Is Part Of: FEBS letters. Volume 588:Issue 23(2014)
• Page Start: 4497
• Page End: 4503
• Publication Date: 2014-10-27
• Subjects: MiRNA -- microRNA -- HF -- healthy follicle -- EAF -- early atretic follicle -- PAF -- progressively atretic follicle -- PI -- propidiumiodide -- FCM -- flow cytometry -- Pig -- MiR-92a -- Smad7 -- Granulosa cell apoptosis -- RNA interference; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.10.021
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Smad7 has a key role in apoptosis of mammalian ovarian granulosa cells (GCs), as it antagonizes and fine‐tunes transforming growth factor β (TGFβ) signaling. This study demonstrates that miR‐92a regulates GC apoptosis in pig ovaries by targeting Smad7 directly. The expression level of miR‐92a was down‐regulated in atretic porcine follicles, whereas miR‐92a expression led to inhibition of GC apoptosis. The Smad7 gene was identified as a direct target of miR‐92a using a dual‐luciferase reporter assay. Transfection of GCs with miR‐92a mimics decreased Smad7 mRNA and protein levels, whereas expression of an miR‐92a inhibitor in GCs had the opposite effect. In addition, knockdown of Smad7 prevented GC apoptosis in cells that expressed the miR‐92a inhibitor. Abstract : MiR‐92a inhibits porcine granulosa cell apoptosis. Smad7 gene is a target of miR‐92a. MiR‐92a inhibits porcine granulosa cell apoptosis by targeting Smad7 gene. Knockdown of Smad7 could prevent miR‐92a inhibitor‐induced GC apoptosis.