MiR‐204 functions as a tumor suppressor by regulating SIX1 in NSCLC. Issue 20 (23rd August 2014)

• Record Type: Journal Article
• Authors: Xia, Yang; Zhu, Yi; Ma, Teng; Pan, Chunfeng; Wang, Jun; He, Zhicheng; Li, Zhi; Qi, Xiaotong; Chen, Yijiang
• Is Part Of: FEBS letters. Volume 588:Issue 20(2014)
• Page Start: 3703
• Page End: 3712
• Publication Date: 2014-08-23
• Subjects: miR-204 -- SIX1 -- Proliferation -- Invasion -- Epithelial-to-mesenchymal transition -- Non-small cell lung cancer; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.08.016
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The involvement of miR‐204 in lung cancer development is unclear. In our study, we analyzed the expression of miR‐204 in tumor‐ and adjacent‐tissue samples from 141 patients with non‐small cell lung cancer (NSCLC). MiR‐204 expression was decreased in tumor samples compared with non‐cancerous tissue‐derived controls. Moreover, miR‐204 expression negatively correlated with homeobox protein SIX1 expression, tumor size and metastasis. MiR‐204 silencing in miR‐204‐positive NSCLC cell lines promoted cell invasion and proliferation. Concomitantly, MiR‐204 overexpression resulted in reduced cell proliferation and invasion, upregulated E‐cadherin and downregulated N‐cadherin and Vimentin expression. SIX1 was identified as a potential target of miR‐204, and SIX1 silencing partially compromised the invasive and proliferative capacity of miR‐204‐deficient cells. Thus, miR‐204 may be involved in the NSCLC development. Abstract : Expression of miR‐204 was decreased in lung cancer tissues. miR‐204 regulated the invasion and proliferation of lung cancer cell lines. Direct binding of miR‐204 to the SIX1 3′‐UTR was confirmed. Aberrant expression of miR‐204 regulated the invasion and proliferation of NSCLC by targeting SIX1.