Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR‐26b‐5p. Issue 12 (8th May 2014)

• Record Type: Journal Article
• Authors: Wu, Tangwei; Chen, Weiqun; Liu, Shuiyi; Lu, Hongda; Wang, Hui; Kong, Deyong; Huang, Xiaodong; Kong, Qinzhi; Ning, Yong; Lu, Zhongxin
• Is Part Of: FEBS letters. Volume 588:Issue 12(2014)
• Page Start: 2107
• Page End: 2114
• Publication Date: 2014-05-08
• Subjects: miR-26b-5p -- Huaier -- Lung cancer -- EZH2 -- Proliferation -- Apoptosis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.04.044
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Various studies have reported that Huaier possesses anti‐tumor effects. However, the mechanisms are not completely elucidated. Here, we found 66 differentially expressed miRNAs in Huaier‐treated pulmonary adenocarcinoma A549 cells, with upregulation of miR‐26b‐5p. Transfection of A549 cells with miR‐26b‐5p mimic inhibited proliferation and induced apoptosis, while transfection of Huaier‐treated A549 cells with a miR‐26b‐5p inhibitor reversed the effects of Huaier. EZH2 was verified as the target of miR‐26b‐5p. Thus, our findings indicate that Huaier might suppress proliferation and induce apoptosis in lung cancer cells via a miR‐26b‐5p‐EZH2‐mediated approach, which provides a new perspective for understanding the anti‐tumor effects of Huaier. Abstract : Huaier can regulate miRNA expression in lung cancer cells. miR‐26b‐5p inhibits proliferation and induces apoptosis in A549 cells. Inhibition of miR‐26b‐5p reverses the effects of Huaier. EZH2 is a direct target of miR‐26b‐5p. EZH2 may be involved in the anti‐tumor effects of Huaier‐miR‐26b‐5p pathways.