Alt a 1 from Alternaria interacts with PR5 thaumatin‐like proteins. Issue 9 (15th March 2014)

• Record Type: Journal Article
• Authors: Gómez-Casado, Cristina; Murua-García, Amaya; Garrido-Arandia, María; González-Melendi, Pablo; Sánchez-Monge, Rosa; Barber, Domingo; Pacios, Luis F.; Díaz-Perales, Araceli
• Is Part Of: FEBS letters. Volume 588:Issue 9(2014)
• Page Start: 1501
• Page End: 1508
• Publication Date: 2014-03-15
• Subjects: Alt a 1 -- PR5-thaumatin-like protein -- Kiwi-PR5 -- Competitive inhibitor -- Alternaria -- Kiwi; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.02.044
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Alt a 1 is a protein found in Alternaria alternata spores related to virulence and pathogenicity and considered to be responsible for chronic asthma in children. We found that spores of Alternaria inoculated on the outer surface of kiwifruits did not develop hyphae. Nevertheless, the expression of Alt a 1 gene was upregulated, and the protein was detected in the pulp where it co‐localized with kiwi PR5. Pull‐down assays demonstrated experimentally that the two proteins interact in such a way that Alt a 1 inhibits the enzymatic activity of PR5. These results are relevant not only for plant defense, but also for human health as patients with chronic asthma could suffer from an allergic reaction when they eat fruit contaminated with Alternaria . Abstract : Alt a 1 could be detected in the pulp of fruits, without fungus development. Alt 1 can bind to members of PR5 family. Alt a 1 partially inhibits enzymatic activity of PR5. Patients with chronic asthma could suffer from symptoms eating contaminated fruit.