Drosophila melanogaster as a model system to study long‐chain fatty acid amide metabolism. Issue 9 (17th March 2014)

• Record Type: Journal Article
• Authors: Jeffries, Kristen A.; Dempsey, Daniel R.; Behari, Anita L.; Anderson, Ryan L.; Merkler, David J.
• Is Part Of: FEBS letters. Volume 588:Issue 9(2014)
• Page Start: 1596
• Page End: 1602
• Publication Date: 2014-03-17
• Subjects: ATP -- adenosine triphosphate -- CoA -- coenzyme A -- EIC -- extracted ion chromatogram -- FAAH -- fatty acid amide hydrolase -- HPLC -- high performance liquid chromatography -- LC/QTOF-MS -- liquid chromatography/quadrupole time-of-flight mass spectrometry -- NAE -- N-acylethanolamine -- PFAM -- primary fatty acid amide -- D. melanogaster -- Drosophila melanogaster -- PAL -- peptidyl-α-hydroxyglycine-α-amidating lyase -- PAM -- peptidylglycine α-amidating monooxygenase -- PHM -- peptidylglycine-α-hydroxylating monooxygenase -- TIC -- total ion chromatogram -- Long-chain fatty acid amide -- Endogenous quantification -- Biosynthetic pathway -- Model system -- Heavy-labeled precursor -- Drosophila melanogaster; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.02.051
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Long‐chain fatty acid amides are cell‐signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long‐chain fatty acid amide metabolism as we have quantified the endogenous levels of N ‐acylglycines, N‐ acyldopamines, N ‐acylethanolamines, and primary fatty acid amides by LC/QTOF‐MS. Growth of D. melanogaster on media supplemented with [1‐ 13 C]‐palmitate lead to a family of 13 C‐palmitate‐labeled fatty acid amides in the fly heads. The [1‐ 13 C]‐palmitate feeding studies provide insight into the biosynthesis of the fatty acid amides. Abstract : Endogenous long‐chain fatty acid amides were identified in D. melanogaster . Long‐chain fatty acid amides were quantified in D. melanogaster using LC/QTOF‐MS. Heavy‐labeled precursors were fed to D. melanogaster . A model system for studying the biosynthesis of fatty acid amides was established.