The PGRS domain is responsible for translocation of PE_PGRS30 to cell poles while the PE and the C‐terminal domains localize it to the cell wall. Issue 6 (11th February 2014)

• Record Type: Journal Article
• Authors: Chatrath, Shweta; Gupta, Vineet Kumar; Garg, Lalit C.
• Is Part Of: FEBS letters. Volume 588:Issue 6(2014)
• Page Start: 990
• Page End: 994
• Publication Date: 2014-02-11
• Subjects: PE_PGRS30 -- Localization -- PE domain -- PGRS domain -- C-terminal domain -- Mycobacterium tuberculosis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.01.059
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : PE_PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE_PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE_PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C‐terminal domain present in few PE_PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE_PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE_PGRS30. Abstract : The role of PE_PGRS30 domains in protein localization. C‐terminal domain possesses individual cell wall localization signals. PGRS domain is exposed on cell surface. Polar localization is due to PGRS domain.