Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis). Issue 6 (11th February 2014)

• Record Type: Journal Article
• Authors: Cankar, Katarina; van Houwelingen, Adèle; Goedbloed, Miriam; Renirie, Rokus; de Jong, René M.; Bouwmeester, Harro; Bosch, Dirk; Sonke, Theo; Beekwilder, Jules
• Is Part Of: FEBS letters. Volume 588:Issue 6(2014)
• Page Start: 1001
• Page End: 1007
• Publication Date: 2014-02-11
• Subjects: CnVO -- Callitropsis nootkatensis valencene oxidase -- CnVS -- Callitropsis nootkatensis valencene synthase -- EST -- expressed sequence tag -- Sesquiterpene -- Cytochrome P450 -- Alaska cedar -- (+)-Valencene -- (+)-Nootkatone -- Callitropsis nootkatensis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.01.061
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : (+)‐Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar ( Callitropsis nootkatensis ) heartwood essential oil. Upon co‐expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans ‐nootkatol and (+)‐nootkatone. Formation of (+)‐nootkatone was detected at 144 ± 10 μg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases. Abstract : (+)‐Nootkatone is a major sesquiterpene component of Alaska cedar essential oil. Alaska cedar valencene oxidase CYP706M1 was identified and cloned. CYP706M1 catalyses the oxidation of (+)‐valencene in yeast and in vitro . (+)‐Nootkatone was produced at 144μg/L by CYP706M1‐expressing yeast culture. CYP706M1 is the first valencene oxidase from a (+)‐nootkatone producing plant.