Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots. Issue 6 (13th February 2014)

• Record Type: Journal Article
• Authors: Ordoñez, Natalia Maria; Marondedze, Claudius; Thomas, Ludivine; Pasqualini, Stefania; Shabala, Lana; Shabala, Sergey; Gehring, Chris
• Is Part Of: FEBS letters. Volume 588:Issue 6(2014)
• Page Start: 1008
• Page End: 1015
• Publication Date: 2014-02-13
• Subjects: Plant stress -- Hydrogen peroxide -- Cyclic mononucleotide -- cAMP -- cGMP -- Ion flux -- Proteomic -- Arabidopsis thaliana; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.01.062
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2 O2 ) induces rapid net K + ‐efflux and Ca 2+ ‐influx in Arabidopsis roots. Pre‐treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K + ‐leakage and Ca 2+ ‐influx, and in the case of the K + ‐fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8‐Br‐cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2 O2 . Abstract : We show that H2 O2 modulates potassium and calcium net fluxes in Arabidopsis roots. We examine the effect of cyclic mononucleotide pre‐treatment on these fluxes. We also investigate the effect of cGMP on the microsomal proteome. cGMP is sufficient to induce complex cellular changes to responses induced by H2 O2 .