Development and validation of an ESI‐LC‐MS/MS method for simultaneous identification and quantification of 24 analytes of forensic relevance in vitreous humour, whole blood and plasma1. Issue 1 (27th April 2015)

• Record Type: Journal Article
• Authors: Arora, Beauty; Velpandian, Thirumurthy; Saxena, Rohit; Lalwani, Sanjeev; Dogra, T. D; Ghose, Supriyo
• Is Part Of: Drug testing and analysis. Volume 8:Issue 1(2016:Jan.)
• Page Start: 87
• Page End: 98
• Publication Date: 2015-04-27
• Subjects: vitreous humour -- ESI‐LC‐MS/MS -- post‐mortem -- drugs, forensic; Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.1797
• Languages: English
• ISSNs: 1942-7603

Abstract

Abstract : Detection and quantification of drugs from various biological matrices are of immense importance in forensic toxicological analysis. Despite the various reported methods, development of a new method for the detection and quantification of drugs is still an active area of research. However, every method and biological matrix has its own limitation, which further encourage forensic toxicologists to develop new methods and to explore new matrices for the analysis of drugs. In this study, an electrospray ionization‐liquid chromatograph‐tandem mass spectrometry (ESI‐LC‐MS/MS) method is developed and validated for simultaneous identification and quantification of 24 drugs of forensic relevance in various body fluids, namely, whole blood, plasma and vitreous humour. The newly developed method has been validated for intra‐day and inter‐day accuracy, precision, selectivity and sensitivity. Absolute recovery shows a mean of 84.5, 86.2, and 103% in the vitreous humour, whole blood and plasma respectively, which is suitable for the screening procedure. Further, the absolute matrix effect (AME) shows a mean of 105, 96.5, and 109% in the vitreous humour, whole blood and plasma, respectively. In addition, to examine the practical utility of this method, it has been applied for screening of drugs in post‐mortem samples of the vitreous humour, whole blood and plasma collected at autopsy from ten cadavers. Experimental results show that the newly developed method is well applicable for screening of analytes in all the three matrices. Copyright © 2015 John Wiley & Sons, Ltd. Abstract : A method is developed and validated for simultaneous screening and quantification of 24 analytes of forensic relevance in vitreous humour, blood and plasma using LC‐MS/MS. The method is advantageous over other methods due to its simplified sample preparation protocol and less sample requirement. The method is well applied for screening of drugs in post‐mortem samples collected at autopsy.