Determination of salvianolic acid C in rat plasma using liquid chromatography–mass spectrometry and its application to pharmacokinetic study. (19th August 2015)

• Record Type: Journal Article
• Authors: Song, Junke; Zhang, Wen; Sun, Jialin; Zhang, Xue; Xu, Xiaona; Zhang, Li; Feng, Zhangying; Du, Guanhua
• Is Part Of: Biomedical chromatography. Volume 30:Number 3(2016:Mar.)
• Page Start: 376
• Page End: 383
• Publication Date: 2015-08-19
• Subjects: salvianolic acid C -- LC–MS -- pharmacokinetics -- bioavailability -- dose proportionality; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3558
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: A sensitive and reliable LC‐ESI‐MS method for the determination of salvianolic acid C in rat plasma has been developed and validated. Plasma samples were prepared by liquid–liquid extraction with ethyl acetate and separated on a Zorbax SB‐C18 column (3.5 µm, 2.1 × 100 mm) at a flow rate of 0.3 mL/min using acetonitrile–water as mobile phase. The detection was carried out by a single quadrupole mass spectrometer with electrospray ionization source and selected ion monitoring mode. Linearity was obtained for salvianolic acid C ranging from 5 to 1000 ng/mL. The intra‐ and inter‐day precisions (RSD, %) didn't exceed 9.96%, and the accuracy (RE, %) were all within ±3.64%. The average recoveries of the analyte and internal standard were >89.13%. Salvianolic acid C was proved to be stable during all sample storage, preparation and analytic procedures. The validated method was successfully applied to pharmacokinetic study after oral and intravenous administration of salvianolic acid C to rats. The absolute oral bioavailability of salvianolic acid C was 0.29 ± 0.05%. This method was further applied to simultaneous determination of salvianolic acid A, salvianolic acid B and salvianolic acid C in rat plasma and showed good practicability. Copyright © 2015 John Wiley & Sons, Ltd.