Disposable microcolumns with welded metal frits. Issue 2 (22nd December 2015)

• Record Type: Journal Article
• Authors: Hong, Sung Hoon; Cheong, Won Jo
• Is Part Of: Journal of separation science. Volume 39:Issue 2(2016)
• Page Start: 243
• Page End: 246
• Publication Date: 2015-12-22
• Subjects: Disposable microcolumns -- Frit welding -- Micro‐welding -- Silica particles; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201501055
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : This study reports the preparation of disposable microcolumns with welded metal frits for the first time. First, the bottom of glass‐lined stainless‐steel tubing of 30 cm length, 1.6 mm od, and 0.5 mm id was welded with a stainless‐steel screen frit of 1.6 mm diameter. A micro‐welding machine was used for this. Next, the column was connected to a slurry packer and packed with porous silica particles. Then, the inlet of the column was carefully welded with another frit. The column was tested for separation of a test mix composed of phenol, 2‐nitrophenol, acetophenone, aceanilide, and benzamide. Another column of the same physical dimension was also prepared with frits that were not welded to the column. The chromatographic performances of the two groups of columns (welded frits versus non‐welded frits) were examined. The columns of welded frits showed ca. 18% better separation efficiency (number of theoretical plates) than those of non‐welded frits.