Isolation and structural elucidation of a novel homogenous polysaccharide from Tricholoma matsutake. Issue 1 (2nd January 2016)

• Record Type: Journal Article
• Authors: Cheng, Hua; Jia, Yan; Wang, Ling; Liu, Xiaoying; Liu, Guangrong; Li, Li; He, Congfen
• Is Part Of: Natural product research. Volume 30:Issue 1(2016)
• Page Start: 58
• Page End: 64
• Publication Date: 2016-01-02
• Subjects: polysaccharide -- Tricholoma matsutake -- GC-MS -- 2D-NMR -- structure elucidation; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2015.1034711
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract : A crude polysaccharide possessing antitumour, radiation-resistant and anti-ageing attributes was extracted from Tricholoma matsutake by water extraction and alcohol precipitation. From this crude polysaccharide, a homogeneous polysaccharide, TMP-5II, was successfully purified by Sephacryl S-300 column chromatography. The average molecular weight ( M w ) of TMP-5II was 15.76 kDa. Monosaccharide analysis indicated that the homogeneous polysaccharide contained four different residues:d -glucose, d -galactose, d -mannose andd -fucose. Attenuated total reflectance infrared spectroscopy revealed characteristics typical of carbohydrate polymers and a peak typical of a β-type glycosidic bond. TMP-5II was selected for structural characterisation by nuclear magnetic resonance (NMR) analysis. According to 1 H NMR, 13 C NMR and two-dimensional-NMR analysis, TMP-5II contains two kinds of linkages, β and α, at a ratio of 4:1. Preliminary results indicated that the polysaccharide had (1-4)-beta-pyran glucose as the main chain, and a branched chain in the O-6 location with fucose (1-2) mannose (1-3)-alpha-pyran galactose. Abstract :