Preparation and evaluation of a molecularly imprinted sol–gel material as the solid‐phase extraction adsorbents for the specific recognition of cloxacilloic acid in cloxacillin. Issue 3 (7th January 2016)

• Record Type: Journal Article
• Authors: Du, Kangli; Luo, Zhimin; Guo, Pengqi; Tang, Weili; Wu, Ningli; Zheng, Penglei; Du, Wei; Zeng, Aiguo; Jing, Wanghui; Chang, Chun; Fu, Qiang
• Is Part Of: Journal of separation science. Volume 39:Issue 3(2016)
• Page Start: 483
• Page End: 489
• Publication Date: 2016-01-07
• Subjects: Allergenic impurities -- Cloxacillin -- Molecularly imprinted polymers -- Sol‐gel process -- Solid‐phase extraction; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201500961
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : Highly selective molecularly imprinted polymers on the surface of silica gels were prepared by a sol–gel process and used as solid‐phase extraction adsorbents for the specific recognition, enrichment and detection of cloxacilloic acid in cloxacillin. The obtained polymers were characterized by scanning electron microscopy, FTIR spectroscopy, nitrogen adsorption and desorption, elemental analysis and thermogravimetric analysis. The imprinted polymers not only possessed high adsorption capacity (6.5 μg/mg), but also exhibited fast adsorption kinetics (they adsorb 80% of the maximum amount within 20 min) and excellent selectivity (the imprinted factor was 3.6). A method using the imprinted polymers as solid‐phase extraction adsorbents coupled with high‐performance liquid chromatography was established with good specificity, linearity ( r = 0.9962), precision (ranging from 0.5 to 6.7%), accuracy (ranging from 93.9 to 97.7%) and extraction recoveries (ranging from 78.8 to 89.8%). The limits of detection and quantification were 0.07 and 0.25 mg/g, respectively. This work could provide a promising method in the enrichment, extraction and detection of allergenic impurities in the manufacture, storage and application of cloxacillin.