Synthesis and characterization of PMMA composites activated with starch for immobilization of L‐asparaginase. Issue 19 (5th February 2016)

• Record Type: Journal Article
• Authors: Ulu, Ahmet; Koytepe, Suleyman; Ates, Burhan
• Is Part Of: Journal of applied polymer science. Volume 133:Issue 19(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-02-05
• Subjects: biodegradable -- biomedical applications -- biopolymers and renewable polymers -- emulsion polymerization; Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.43421
• Languages: English
• ISSNs: 0021-8995

Abstract

ABSTRACT: Poly (methyl methacrylate) (PMMA)–starch composites were prepared by emulsion polymerization technique for L‐asparaginase (L‐ASNase) immobilization as highly activated support. The hydroxide groups on the prepared composites offer a very simple, mild and firm combination for enzyme immobilization. The pure PMMA and PMMA‐starch composites were characterized as structural, thermal and morphological. PMMA‐starch composites were found to have better thermal stability and more hydrophilic character than pure PMMA. L‐ASNase was immobilized onto PMMA‐starch composites contained the different ratio of starch (1, 3, 5, and 10 wt %). Immobilized L‐ASNase showed better performance as compared to the native enzyme in terms of thermal stability and pH. Km value of immobilized enzyme decreased approximately eightfold compared with the native enzyme. In addition to, immobilized L‐ASNase was found to retain 60% of activity after 1‐month storage period at 4 °C. Therefore, PMMA‐starch composites can be provided more advantageous in terms of enzymatic affinity, thermal, pH and storage stability as L‐ASNase immobilization matrix. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2016, 133, 43421. Abstract :