CDNA cloning and expression analysis of the juvenile hormone acid methyltransferase from seabuckthorn carpenterworm, Holcocerus hippophaecolus (Lepidoptera: Cossidae). Issue 1 (15th December 2015)

• Record Type: Journal Article
• Authors: Zhang, Shuo; Wang, Yu; Zhou, Jiao; Li, Juan; Zong, Shi‐Xiang; Luo, You‐Qing; Weng, Qiang
• Is Part Of: Entomological research. Volume 46:Issue 1(2016:Jan.)
• Page Start: 23
• Page End: 30
• Publication Date: 2015-12-15
• Subjects: corpora allata -- Holcocerus hippophaecolus -- JHAMT -- juvenile hormone -- methyltransferase; Insects -- Korea -- Periodicals; Entomology -- Periodicals; 595.709519
• DOI: 10.1111/1748-5967.12144
• Languages: English
• ISSNs: 1738-2297

Abstract

Abstract: In this study, we report the cDNA cloning and sequence determination of H h‐ JHAMT from the seabuckthorn carpenterworm, H olcocerus hippophaecolus, by using rapid amplification of cDNA ends. The full‐length cDNA of putative H h‐ JHAMT was 1659 bp and contained a highly conserved Motif I, SAM motif I, which showed that Hh‐JHAMT like enzyme was a member of SAM‐dependent MTases. Moreover, putative Hh‐JHAMT had high homology to the other members of the JHAMT peptide family: 59% with S podoptera litura, 54% with B ombyx mori and 54% with H elicoverpa armigera . Multiple alignments and phylogenetic analysis revealed that H h‐ JHAMT was closely related to JHAMT from Lepidoptera. Real‐time quantitative PCR experiments showed that H h‐ JHAMT mRNA expression was highest in the corpora allata (CA) complex, and was also detected at high levels during earlier larval and adult stages. The JHAMT mRNA level gradually declined during larval development, and the lowest amount of expression was observed in the pupal stage, while it increased to a higher level during adult stages. The pattern of H h‐ JHAMT expression was similar to the mode of JH biosynthesis. These results provided information concerning molecular characteristics of H h‐ JHAMT, whose expression profile suggests that the H h‐ JHAMT gene might be changed with larval development, metamorphosis and adult reproduction of the H . hippophaecolus.