Investigation of the Viability, Adhesion, and Migration of Human Fibroblasts in a Hyaluronic Acid/Gelatin Microgel‐Reinforced Composite Hydrogel for Vocal Fold Tissue Regeneration. Issue 2 (26th October 2015)

• Record Type: Journal Article
• Authors: Heris, Hossein K.; Daoud, Jamal; Sheibani, Sara; Vali, Hojatollah; Tabrizian, Maryam; Mongeau, Luc
• Is Part Of: Advanced healthcare materials. Volume 5:Issue 2(2016)
• Page Start: 255
• Page End: 265
• Publication Date: 2015-10-26
• Subjects: cell adhesion/spreading -- cell migration mechanisms -- hyaluronic acid/gelatin hydrogel -- vocal fold repair; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201500370
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : The potential use of a novel scaffold biomaterial consisting of cross‐linked hyaluronic acid (HA)–gelatin (Ge) composite microgels is investigated for use in treating vocal fold injury and scarring. Cell adhesion integrins and kinematics of cell motion are investigated in 2D and 3D culture conditions, respectively. Human vocal fold fibroblast (hVFF) cells are seeded on HA–Ge microgels attached to a HA hydrogel thin film. The results show that hVFF cells establish effective adhesion to HA–Ge microgels through the ubiquitous expression of β1 integrin in the cell membrane. The microgels are then encapsulated in a 3D HA hydrogel for the study of cell migration. The cells within the HA–Ge microgel‐reinforced composite hydrogel (MRCH) scaffold have an average motility speed of 0.24 ± 0.08 μm min −1 . The recorded microscopic images reveal features that are presumably associated with lobopodial and lamellipodial cell migration modes within the MRCH scaffold. Average cell speed during lobopodial migration is greater than that during lamellipodial migration. The cells move faster in the MRCH than in the HA–Ge gel without microgels. These findings support the hypothesis that HA–Ge MRCH promotes cell adhesion and migration; thereby they constitute a promising biomaterial for vocal fold repair. Abstract : Cell adhesion integrins and kinematics of cell motion are investigated in a novel hyaluronic acid (HA)–gelatin (Ge) microgel‐reinforced composite hydrogel (MRCH) scaffold. Human fibroblast cells establish effective adhesion to HA–Ge microgels through the ubiquitous expression of β1 integrin in the cell membrane. The cells within the MRCH have an average motility speed of 0.24 ± 0.08 μm min −1 .