Emergence of cutaneous neosporosis in a dog receiving immunosuppressive therapy: molecular identification and management. Issue 1 (1st December 2015)

• Record Type: Journal Article
• Authors: Legnani, Sara; Pantchev, Nikola; Forlani, Annalisa; Zini, Eric; Schares, Gereon; Balzer, Jörg; Roccabianca, Paola; Ferri, Filippo; Zanna, Giordana
• Is Part Of: Veterinary dermatology. Volume 27:Issue 1(2016:Feb.)
• Page Start: 49
• Page End: e14
• Publication Date: 2015-12-01
• Subjects: Veterinary dermatology -- Periodicals; Pet medicine -- Periodicals; 636.08965
• DOI: 10.1111/vde.12273
• Languages: English
• ISSNs: 0959-4493

Abstract

Abstract : Background: Neosporosis is a multisystemic disease caused by the intracellular protozoan Neospora caninum . In dogs the disease primarily affects the central nervous system. Canine cutaneous neosporosis is a rare condition often associated with old age or concurrent immunosuppressive treatments for different underlying conditions. Animals: A 10‐year‐old female spayed golden retriever dog affected by primary immune‐mediated myelofibrosis and treated with immunosuppressive therapies for 6 weeks that developed severe cutaneous lesions. Methods: Definitive diagnosis was based on several investigation techniques including serology (immunoblotting), immunohistochemistry (IHC), species‐specific conventional and real‐time PCR, and DNA sequencing. Results: Remission of cutaneous neosporosis was obtained with the administration of clindamycin while the concurrent immunosuppressive therapy was maintained to manage the underlying primary condition. Conclusions and clinical importance: To the best of the authors' knowledge this is the first report of species‐specific PCR and DNA sequencing used as diagnostic methods for canine cutaneous neosporosis emerging in a dog receiving immunosuppressive therapy. Abstract : Background – Neosporosis is a multisystemic disease caused by the intracellular protozoan Neospora caninum . In dogs the disease primarily affects the central nervous system. Canine cutaneous neosporosis is a rare condition often associated with old age or concurrent immunosuppressive treatments for different underlying conditions.Conclusions and clinical importance – To the best of the authors' knowledge this is the first report of species‐specific PCR and DNA sequencing used as diagnostic methods for canine cutaneous neosporosis emerging in a dog receiving immunosuppressive therapy.